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The Microscopic Diagnosis of Malaria

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Course Introduction   |   Overview   |   Plasmodium falciparum   |   Plasmodium vivax

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Lesson 2 Plasmodium falciparum Trophozoites, Thin Film

overstained thin film Plasmodium falciparum trophozoites

Is this smear positive or negative?

 

This is an alkaline buffer with overstained RBCs. It is positive, but some of the parasites have nuclei that are almost black, making it difficult to discern whether the colors red and blue are present.

 

If you look at the arrow on the far right, you will see a typical trophozoite of P. falciparum. Notice the characteristic half ring with two nuclei.  

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